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M94A3319.TXT
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1994-10-25
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Document 3319
DOCN M94A3319
TI HIV-1 production from unintegrated provirus in transfected Jurkat
clones.
DT 9412
AU Gurgo C; Zella D; Reitz MS Jr; Gallo RC; Laboratory of Tumor Cell
Biology, NCI NIH, Bethesda, MD.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):103 (abstract no. PA0030). Unique
Identifier : AIDSLINE ICA10/94369256
AB Production of viral antigens and infectious virus by Jurkat cells
transfected with pHXB2 (nef+) progressively declined over an extended
period of time in culture. Sixty clones were generated from the
transfected line; they were analyzed for the presence of provirus and
monitored for the expression of viral antigens (surface gp120 and p24)
and CD4. Six/60 clones were positive for the release of p24. Circular
viral DNA was present in all p24 positive clones and absent in all p24
negative clones (54/60). In addition to circular DNA, 5/6 p24+ clones
contain an altered form of the original construct. The remaining p24+
clone had the original pHXB2 gpt construct, as well as an altered form
of the construct; it releases high levels of p24. All the p24- clones
(54/60) contained altered forms of pHXB2 (but not the original
construct). No clonally integrated provirus could be detected in any of
the clones, suggesting that the clonal progenitor cell did not contain
an integrated provirus. Thus, in the absence of integration, virus may
persist in an unintegrated form, yet still be expressed and result in an
active infection.
DE Cell Line Clone Cells/MICROBIOLOGY Genes, nef Human HIV
Antigens/BIOSYNTHESIS HIV Core Protein p24/BIOSYNTHESIS
HIV-1/GENETICS/IMMUNOLOGY/*PHYSIOLOGY Proviruses/GENETICS/*PHYSIOLOGY
Transfection Virus Integration/GENETICS/PHYSIOLOGY Virus
Replication/GENETICS/PHYSIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).